期刊
PLOS ONE
卷 15, 期 12, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0244713
关键词
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资金
- project PhageSTEC - FEDER through COMPETE2020 (Programa Operacional Competitividade e Internacionalizacao) [POCI-01-0145 -FEDER-029628]
- FCT under the European Regional Development Fund (Norte2020 -Programa Operacional Regional do Norte) [UIDB/04469/2020, NORTE-01-0145-FEDER-000004]
- Plan Estatal de I+D+I 2013-2016, Instituto de Salud Carlos III (ISCIII), Subdireccion General de Evaluacion y Fomento de la Investigacion, Ministerio de Economia y Competitividad (Gobierno de Espana) [PI16/01477]
- FEDER
- Conselleria de Cultura, Educacion e Ordenacion Universitaria, (Xunta de Galicia) [ED431C2017/57]
- FCT [UIDB/AGR/04033/2020]
- Conselleria de Cultura, Educacion e Ordenacion Universitaria, Xunta de Galicia [ED481A-2015/149]
- FPU programme from Secretaria General de Universidades, Spanish Ministerio de Educacion, Cultura y Deporte [FPU15/02644]
- FCT (Fundacao para a Ciencia e a Tecnologia)
The prevalence of Shiga toxin (Stx)-producing Escherichia coli (STEC) was determined by evaluating its presence in faecal samples from 155 heifers, and 254 dairy cows in 21 farms at North of Portugal sampled between December 2017 and June 2019. The prevalence of STEC in heifers (45%) was significantly higher than in lactating cows (16%) (p<0.05, Fisher exact test statistic value is <0.00001). A total of 133 STEC were isolated, 24 (13.8%) carried Shiga-toxin 1 (stx1) genes, 69 (39.7%) carried Shiga-toxin 2 (stx2) genes, and 40 (23%) carried both stx1 and stx2. Intimin (eae) virulence gene was detected in 29 (21.8%) of the isolates. STEC isolates belonged to 72 different O:H serotypes, comprising 40 O serogroups and 23 H types. The most frequent serotypes were O29:H12 (15%) and O113:H21 (5.2%), found in a large number of farms. Two isolates belonged to the highly virulent serotypes associated with human disease O157:H7 and O26:H11. Many other bovine STEC serotypes founded in this work belonged to serotypes previously described as pathogenic to humans. Thus, this study highlights the need for control strategies that can reduce STEC prevalence at the farm level and, thus, prevent food and environmental contamination.
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