期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 160, 期 -, 页码 404-412出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2021.01.023
关键词
Endophytic fungal elicitor; Fatty acid; Amino acid; Primary metabolism; Mucor fragilis
资金
- National Natural Science Foundation of China [81773841]
- Science & Technology Support Plan of Guizhou Province [20172546]
- Science &Technology Support Plan of Guizhou Province [20204Y074]
The study revealed that the endophytic fungus Mucor fragilis functions as an effective elicitor for improving the accumulation of primary and secondary metabolites in Salvia miltiorrhiza hairy roots by regulating gene expression. This finding provides guidance for quality control of S. miltiorrhiza and highlights the potential application of Mucor fragilis as an effective endophytic fungal elicitor.
Salvia miltiorrhiza is one of the most commonly used medicinal materials in China. In recent years, the quality of S. miltiorrhiza has attracted much attention. Biotic and abiotic elicitors are widely used in cultivation to improve the quality of medicinal plants. We isolated an endophytic fungus, Mucor fragilis, from S. miltiorrhiza. We compared the effects of endophytic fungal elicitors with those of yeast extract together with silver ion, widely used together as effective elicitors, on S. miltiorrhiza hairy roots. Seventeen primary metabolites (amino acids and fatty acids) and five secondary metabolites (diterpenoids and phenolic acids) were analyzed after elicitor treatment. The mycelium extract promoted the accumulation of salvianolic acid B, rosmarinic acid, stearic acid, and oleic acid in S. miltiorrhiza hairy roots. Additionally, qPCR revealed that elicitors affect the accumulation of primary and secondary metabolites by regulating the expression of key genes (SmAACT, SmGGPPS, and SmPAL). This is the first detection of both the primary and secondary metabolites of S. miltiorrhiza hairy roots, and the results of this work should help guide the quality control of S. miltiorrhiza. In addition, the findings confirm that Mucor fragilis functions as an effective endophytic fungal elicitor with excellent application prospect for cultivation of medicinal plants.
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