Chemical defence in Brassicaceae against pollen beetles revealed by metabolomics and flower bud manipulation approaches

Divergence of chemical plant defence mechanisms within the Brassicaceae can be utilized to identify means against specialized pest insects. Using a bioassay-driven approach, we (a) screened 24 different Brassica napus cultivars, B. napus resyntheses and related brassicaceous species for natural plant resistance against feeding adults of the pollen beetle (Brassicogethes aeneus), (b) tested for gender-specific feeding resistance, (c) analysed the flower bud metabolomes by a non-targeted approach and (d) tested single candidate compounds for their antifeedant activity. (a) In no-choice assays, beetles were allowed to feed on intact plants. Reduced feeding activity was mainly observed on Sinapis alba and Barbarea vulgaris but not on B. napus cultivars. (b) Males fed less and discriminated more in feeding than females. (c) Correlation of the metabolite abundances with the beetles' feeding activity revealed several glucosinolates, phenylpropanoids, flavonoids and saponins as potential antifeedants. (d) These were tested in dual-bud-choice assays developed for medium-throughput compound screening. Application of standard compounds on single oilseed rape flower buds revealed highly deterrent effects of glucobarbarin, oleanolic acid and hederagenin. These results help to understand chemical plant defence in the Brassicaceae and are of key importance for further breeding strategies for insect-resistant oilseed rape cultivars.

Automated summary

The divergence of chemical plant defense mechanisms within the Brassicaceae family can be utilized to identify strategies against specialized pest insects. Screening different Brassica napus cultivars and related species revealed potential antifeedant compounds such as glucosinolates and phenylpropanoids. This research provides insights into plant defense mechanisms and is crucial for developing breeding strategies for insect-resistant oilseed rape cultivars.