4.7 Article

Autophagy Contributes to the Quality Control of Leaf Mitochondria

期刊

PLANT AND CELL PHYSIOLOGY
卷 62, 期 2, 页码 229-247

出版社

OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcaa162

关键词

Arabidopsis (Arabidopsis thaliana); Autophagy; Mitochondria; Mitophagy; Organelle quality control; Ultraviolet B

资金

  1. Japan Society for the Promotion of Science (JSPS) KAKENHI [JP16H06280, JP17H05050, JP18H04852, JP19H04712, JP20H04916, JP20K21322, JP19J01681, JP20K15501, JP20H05352, JP20H05306, JP17H06350]
  2. JSPS Research Fellowship
  3. Japan Science and Technology Agency (JST) PRESTO [JPMJPR16Q1]
  4. Joint Research by Exploratory Research Center on Life and Living Systems (ExCELLS Program) [20-314]
  5. Cooperative Research Program of 'NJRC Mater. Dev.'

向作者/读者索取更多资源

Autophagy is involved in plant tolerance to the photooxidative stress caused by ultraviolet B (UVB) radiation, with mitophagy serving as a process for maintaining mitochondrial quality by eliminating dysfunctional mitochondria. Following a 1-h UVB exposure, an increase in autophagosome formation and active transport of mitochondria into the central vacuole were observed. Autophagy also plays a role in removing depolarized mitochondria when mitochondrial function is disrupted, contributing to mitochondrial quality control in Arabidopsis leaves.
In autophagy, cytoplasmic components of eukaryotic cells are transported to lysosomes or the vacuole for degradation. Autophagy is involved in plant tolerance to the photooxidative stress caused by ultraviolet B (UVB) radiation, but its roles in plant adaptation to UVB damage have not been fully elucidated. Here, we characterized organellar behavior in UVB-damaged Arabidopsis (Arabidopsis thaliana) leaves and observed the occurrence of autophagic elimination of dysfunctional mitochondria, a process termed mitophagy. Notably, Arabidopsis plants blocked in autophagy displayed increased leaf chiorosis after a 1-h UVB exposure compared to wild-type plants. We visualized autophagosomes by labeling with a fluorescent protein-tagged autophagosome marker, AUTOPHAGY8 (ATG8), and found that a 1-h UVB treatment led to increased formation of autophagosomes and the active transport of mitochondria into the central vacuole. In atg mutant plants, the mitochondrial population increased in UVB-damaged leaves due to the cytoplasmic accumulation of fragmented, depolarized mitochondria. Furthermore, we observed that autophagy was involved in the removal of depolarized mitochondria when mitochondrial function was disrupted by mutation of the FRIENDLY gene, which is required for proper mitochondrial distribution. Therefore, autophagy of mitochondria functions in response to mitochondrion-specific dysfunction as well as UVB damage. Together, these results indicate that autophagy is centrally involved in mitochondrial quality control in Arabidopsis leaves.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据