期刊
PIGMENT CELL & MELANOMA RESEARCH
卷 34, 期 3, 页码 575-584出版社
WILEY
DOI: 10.1111/pcmr.12956
关键词
cyclobutane dimers; DNA damage; DNA repair; melanin; skin type
资金
- Walgreens Boots Alliance
This study found the presence of dark CPD in human skin, which may be influenced by melanin location and concentration. Repair kinetics of different skin types show no significant differences in CPD formation and repair post-exposure.
Introduction: Unlike light cylobutane pyrimidine dimers (CPD) formed during ultraviolet radiation (UVR) exposure, dark CPD (dCPD) are formed afterwards. Studies have attributed this to delayed melanin sensitization. There are no data on the role of melanin in dCPD formation in human skin. Methods and Results: Volunteers of Fitzpatrick skin types (FST I/II vs. VI) were exposed to erythemally equivalent doses of solar simulated radiation. CPD were assessed by semi-quantitative immunostaining in whole epidermis and in three epidermal zones, and quantitative HPLC-MS/MS (whole epidermis) at different times post-exposure up to 24 hr. A CPD peak that appeared at 1-2 hr post-exposure in whole epidermis measurements, in all skin types, demonstrated dCPD. However, both dCPD and light CPD were absent in the basal layer of FST VI with the greatest melanin concentration. Modelling the whole epidermis data showed no differences between the repair kinetics of FST I/II and VI. Discussion: Melanin may be a sensitizer or sunscreen for dCPD depending on its location and concentration. Previous CPD repair studies in human skin have assumed peak CPD immediately after UVR exposure and so have overestimated total repair.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据