期刊
PHYTOTHERAPY RESEARCH
卷 35, 期 5, 页码 2807-2823出版社
WILEY
DOI: 10.1002/ptr.7026
关键词
fibroblast-like synovial cells; glycosides of Caulis Lonicerae; helper T cells; inflammatory proliferation; Lonicera japonica
资金
- State Administration of Traditional Chinese Medicine of the People's Republic of China [JDZX2015191]
The study evaluated the mechanism of glycosides from Caulis Lonicerae in inhibiting inflammatory proliferation of FLS induced by IL-1β, finding that it restored T cell balance and suppressed JAK-STAT and NF-kappa B signaling pathways.
Caulis Lonicerae, the dried stem of Lonicera japonica, has been confirmed to have antiinflammatory and antioxidant therapeutic effects. In the present study, we aimed to evaluate the functional mechanism of glycosides extracted from Caulis Lonicerae on the inflammatory proliferation of interleukin-1 beta (IL-1 beta)-mediated fibroblast-like synoviocytes (FLSs) from rats. Rat FLSs (RSC-364) co-cultured with lymphocytes induced by IL-1 beta were used as a cell model. Glycosides in a freeze-dried powder of aqueous extract from Caulis Lonicerae were identified using high-performance liquid chromatography-electrospray ionization/mass spectrometry. After treatment with glycosides, the inflammatory proliferation of FLS, induced by IL-1 beta, decreased significantly. Flow cytometry analysis showed that treatment with glycosides restored the abnormal balance of T cells by intervening in the proliferation and differentiation of helper T (Th) cells. Glycosides also inhibited the activation of Janus kinase signal transducer and activator of transcription (JAK-STAT) and nuclear factor (NF)-kappa B signaling pathways by suppressing the protein expression of key molecules in these pathways. Therefore, we concluded that the glycosides of Caulis Lonicerae can intervene in the differentiation of Th cells, suppressing the activation of JAK-STAT and NF-kappa B signaling pathways, contributing to the inhibitory effect on inflammatory proliferation of FLS co-cultured with lymphocytes induced by pro-inflammatory cytokines.
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