Imaging the response to deep brain stimulation in rodent using functional ultrasound

In this study, we explored the feasibility of using functional ultrasound (fUS) imaging to visualize cerebral activation associated with thalamic deep brain stimulation (DBS), in rodents. The ventrolateral (VL) thalamus was stimulated using electrical pulses of low and high frequencies of 10 and 100 Hz, respectively, and multiple voltages (1-7 V) and pulse widths (50-1500 mu s). The fUS imaging demonstrated DBS-evoked activation of cerebral cortex based on changes of cerebral blood volume, specifically at the primary motor cortex (PMC). Low frequency stimulation (LFS) demonstrated significantly higher PMC activation compared to higher frequency stimulation (HFS), at intensities (5-7 V). Whereas, at lower intensities (1-3 V), only HFS demonstrated visible PMC activation. Further, LFS-evoked cerebral activation was was primarily located at the PMC. Our data presents the functionality and feasibility of fUS imaging as an investigational tool to identify brain areas associated with DBS. This preliminary study is an important stepping stone towards conducting real-time functional ultrasound imaging of DBS in awake and behaving animal models, which is of significant interest to the community for studying motor-related disorders.

Automated summary

This study explored the feasibility of using functional ultrasound imaging to visualize cerebral activation associated with thalamic deep brain stimulation in rodents. The results showed that fUS imaging can identify DBS-evoked cerebral cortex activation, with low frequency stimulation having a higher activation effect on the primary motor cortex compared to higher frequencies. This preliminary study is an important step towards conducting real-time functional ultrasound imaging of DBS in awake and behaving animal models.