期刊
PHARMACOLOGICAL RESEARCH
卷 165, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phrs.2021.105436
关键词
Type 2 diabetes; Enteromorpha proliferao; Ligosaccharide; Proteomics; COL1A1
资金
- Key Project of the Natural Science Foundation of Fujian Province [2020J02032]
- Double First-Class Construction Plan of Fujian Agriculture and Forestry University [KSYLX013]
- Key Laboratory of Marine Biotechnology of Fujian Province [2020MB05]
- Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae [2018FZSK01]
A potential new biomarker Col1a1 was identified in this study, showing a link to hypoglycemic activity for T2D and suggesting a novel therapeutic target for alleviating the disease.
Type 2 diabetes (T2D) is a public health problem with a rising incidence worldwide. In this study, a potential new biomarker for T2D and mechanisms underlying the hypoglycemic effects of Enteromorpha prolifera oligosaccharide were investigated. Tandem mass tag labeling with LC-MS/MS was used to identify the differentially expressed proteins (DEPs) between the jejunum of diabetic rats and control rats. Correlations between glycometabolic parameters and DEPs were revealed by a network analysis. The expression levels of target genes in key metabolic pathways were further evaluated to identify candidate biomarkers. Among 6810 total proteins, approximately 88 % were quantified, of which 148 DEPs with a fold change of <0.83 or>1.2 and a corrected p-value of <0.05 were identified. A KEGG enrichment analysis indicated that the hypoglycaemic effects of E. prolifera oligosaccharide involved the PI3K/AKT and extracellular matrix receptor interaction signaling pathways. More importantly, Col1a1 was the most significant gene in the extracellular matrix receptor interaction pathway and was linked to hypoglycaemic activity for the first time. Thus, Col1a1 is a novel potential therapeutic target for alleviating T2D.
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