期刊
PEST MANAGEMENT SCIENCE
卷 77, 期 3, 页码 1536-1547出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/ps.6178
关键词
rice; herbicide; borer; brown planthopper; bacterial blight; rice blast; multigene transformation; pest management
资金
- National Program of Science Research and Development of China [2016YFD0100600]
- National Program of Transgenic Variety Development of China [2016ZX08001-001]
A highly efficient transgene stacking system was used to develop a multiresistance rice variety with improved resistance to glyphosate, borers, brown planthopper, bacterial blight, and rice blast. The obtained rice variety showed desirable agronomic traits and higher yield compared to the recipient cultivar under natural occurrence of pests and diseases.
Background Weeds, diseases and pests pose serious threats to rice production and cause significant economic losses. Cultivation of rice varieties with resistance to herbicides, diseases and pests is believed to be the most economical and environmentally friendly method to deal with these problems. Results In this study, a highly efficient transgene stacking system was used to assembly the synthetic glyphosate-tolerance gene (I. variabilis-EPSPS*), lepidopteran pest resistance gene (Cry1C*), brown planthopper resistance genes (Bph14* and OsLecRK1*), bacterial blight resistance gene (Xa23*) and rice blast resistance gene (Pi9*) onto a transformable artificial chromosome vector. The construct was transferred into ZH11 (a widely used japonica rice cultivar Zhonghua 11) via Agrobacterium-mediated transformation and 'multiresistance rice' (MRR) with desirable agronomic traits was obtained. The results showed that MRR had significantly improved resistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast relative to the recipient cultivar ZH11. Besides, under the natural occurrence of pests and diseases in the field, the yield of MRR was significantly higher than that of ZH11. Conclusion A multigene transformation strategy was employed to successfully develop rice lines with multiresistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast, and the obtained MRR is expected to have great application potential.
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