Regulation of human THP-1 macrophage polarization by Trichinella spiralis

Trichinella spiralis is a foodborne zoonotic nematode, which causes trichinellosis. During the infection, parasite evades the host immune responses by direct and indirect (through excretory-secretory products) contact with host immune cells. One of the main targets for immunomodulation induced by helminths are macrophages. In this study, we examined whether direct contact of different stages of T. spiralis can affect the polarization of human THP-1 macrophages. Co-culture of adult parasite stage and cells in direct contact without LPS addition had a significant impact on TNF alpha levels. Interestingly, in settings with the addition of LPS, the levels of IL-1 beta and TNF alpha significantly increased in adult parasite and newborn larvae (NBL) but not for muscle larvae (ML). While we tested muscle larvae ESP products to compare its effect with whole ML parasite, we detect an increase of pro-inflammatory cytokines like IL-1 beta and TNF alpha in no LPS conditions. Whereas, muscle larvae ESP significantly suppressed the inflammatory response measured by IL-1 beta, TNF alpha, and IL-6 levels and anti-inflammatory IL-10 compared to LPS control. Our findings indicate the anti-inflammatory potential of T. spiralis muscle larvae excretory-secretory products and propose signaling pathways which might be engaged in the mechanism of how muscle larvae ESP affect human macrophages.

Automated summary

This study investigated the impact of direct contact of different stages of T. spiralis on the polarization of human THP-1 macrophages, revealing significant increases in levels of pro-inflammatory cytokines like IL-1 beta and TNF alpha in adult parasite and newborn larvae (NBL). Additionally, muscle larvae excretory-secretory products were found to significantly suppress the inflammatory response while enhancing anti-inflammatory IL-10 levels, suggesting anti-inflammatory potential.