期刊
OSTEOARTHRITIS AND CARTILAGE
卷 29, 期 1, 页码 89-99出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2020.08.002
关键词
Cartilage; IL-1 beta; TRPV4; Mechanotransduction; Hypo-osmolarity; Cilia
资金
- China Scholarship Council
- UK Medical Research Council [MR/L002876/1]
- Biotechnology and Biomedical Sciences Research Council [BB/R003610/1]
- MRC [MR/L002876/1] Funding Source: UKRI
The study demonstrates that TRPV4 plays an anti-inflammatory role in chondrocytes and cartilage explants, regulating tubulin modifications and cilia length to suppress interleukin-1β-mediated inflammatory signaling.
Objective: Cartilage health is maintained in response to a range of mechanical stimuli including compressive, shear and tensile strains and associated alterations in osmolality. The osmotic-sensitive ion channel Transient Receptor Potential Vanilloid 4 (TRPV4) is required for mechanotransduction. Mechanical stimuli inhibit interleukin-1 beta (IL-1 beta) mediated inflammatory signalling, however the mechanism is unclear. This study aims to clarify the role of TRPV4 in this response. Design: TRPV4 activity was modulated glycogen synthase kinase (GSK205 antagonist or GSK1016790 A (GSK101) agonist) in articular chondrocytes and cartilage explants in the presence or absence of IL-1 beta, mechanical (10% cyclic tensile strain (CTS), 0.33 Hz, 24hrs) or osmotic loading (200mOsm, 24hrs). Nitric oxide (NO), prostaglandin E-2 (PGE(2)) and sulphated glycosaminoglycan (sGAG) release and cartilage biomechanics were analysed. Alterations in post-translational tubulin modifications and primary cilia length regulation were examined. Results: In isolated chondrocytes, mechanical loading inhibited IL-1 beta mediated NO and PGE2 release. This response was inhibited by GSK205. Similarly, osmotic loading was anti-inflammatory in cells and ex plants, this response was abrogated by TRPV4 inhibition. In explants, GSK101 inhibited IL-1 beta mediated NO release and prevented cartilage degradation and loss of mechanical properties. Upon activation, TRPV4 cilia localisation was increased resulting in histone deacetylase 6 (HDAC6)-dependent modulation of soluble tubulin and altered cilia length regulation. Conclusion: Mechanical, osmotic or pharmaceutical activation of TRPV4 regulates HDAC6-dependent modulation of ciliary tubulin and is anti-inflammatory. This study reveals for the first time, the potential of TRPV4 manipulation as a novel therapeutic mechanism to supress pro-inflammatory signalling and cartilage degradation. (C) 2020 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International.
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