Epimerization-Free C-Term Activation of Peptide Fragments by Ball Milling

Peptides were produced in high yields and, if any, very low epimerization, by mechanochemical coupling of peptide fragments containing highly epimerization-prone and/or highly hindered amino acids at C-term. Ball milling was clearly identified as the key element enabling one to obtain such results.

Automated summary

Peptides can be produced in high yields with minimal epimerization through mechanochemical coupling of peptide fragments containing highly epimerization-prone and/or highly hindered amino acids at the C-terminus, with ball milling identified as the key element enabling such results.