期刊
ORAL DISEASES
卷 28, 期 1, 页码 142-149出版社
WILEY
DOI: 10.1111/odi.13751
关键词
Andrographolide; chemoprevention; head and neck cancer; leukoplakia; nanoparticles
资金
- National Natural Science Foundation of China [82074502]
- Scientific Research Innovation Projects of Shanghai Jiao Tong University School of Medicine [201813010]
- Medical-Engineering Cross Foundation of Shanghai Jiao Tong University [YG2017MS02]
- Shanghai Xuhui District Medical Research Project [SHXH201718, SHXH201706]
This study demonstrates that ADG-loaded solid lipid nanoparticles (ADG-SLN) exhibit superior inhibitory activity against head and neck cancer and precancerous cells compared with free ADG, mainly due to their higher efficiency of cellular uptake and intracellular absorption.
Background Increasing evidence indicates that andrographolide (ADG) exhibits anti-cancer activity against various cancer cell lines. However, its high hydrophobicity and poor bioavailability restrict its clinical application as a chemopreventative agent. Previously, we have shown that ADG-loaded solid lipid nanoparticles (SLNs) significantly enhanced the bioavailability and anti-hyperlipidemic activity of ADG. Objectives We aimed to investigate whether ADG-SLN enhanced the bioavailability and anti-cancer efficacy of ADG in the human immortalized oral epithelial (HIOEC), precancerous leukoplakia (Leuk1), HN6, and HN30 cells that represented an in vitro model of stepwise head and neck squamous cell carcinoma development. Results The 50% inhibitive concentration (IC50) of ADG-SLN was significantly lower than that of free ADG against HIOEC, Leuk1, and HN6 and HN30 cells. Moreover, ADG-SLN was more effective than free ADG in promoting cell cycle arrest and apoptosis. Importantly, intracellular absorption of ADG was significantly higher in HN6 cells treated with ADG-SLN compared with free ADG-treated cells. Conclusions Our preliminary study demonstrates that ADG-SLN exhibits superior inhibitory activity against head and neck cancer and precancerous cells compared with free ADG. This effect is due to the higher efficiency of cellular uptake and intracellular absorption by ADG-SLN.
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