High-throughput optical sectioning via line-scanning imaging with digital structured modulation

Optical sectioning with high-throughput, a high signal-to-noise ratio (SNR), and submicrometer resolution is crucial, but challenging, to three-dimensional visualization of large biological tissue samples. Here we propose line-scanning imaging with digital structured modulation for optical sectioning. Our method generates images with a significantly improved SNR, compared to wide-field structured illumination microscopy (WF-SIM), without residual modulation artifacts. We image a 14.5 mm x 11.5 mm horizontal view of mouse brain tissue at a pixel resolution of 0.32 mu m x 0.32 mu m in 101 s, which, compared to WF-SIM, represents a significant improvement on imaging throughput. These results provide development opportunities for high-throughput, high-resolution large-area optical imaging methods. (C) 2021 Optical Society of America

Automated summary

This study introduces a new method for optical sectioning imaging that achieves higher image quality and throughput compared to traditional methods. By imaging large biological tissue samples, it opens up new possibilities for the development of high-throughput, high-resolution optical imaging methods.