期刊
ONCOLOGY REPORTS
卷 45, 期 2, 页码 535-546出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2020.7885
关键词
Orf virus; antitumor; colorectal cancer; cytokines; apoptosis
类别
资金
- National Natural Science Foundation of China (NSFC) [81773271, 31672536]
- Key Projects of Basic and Applied Research (Natural Science Class)
- Department of Education, Guangdong Province [2017KZDXM088, 2018KQNCX284]
- Joint Fund of Basic and Applied Basic Research Fund of Guangdong Province [2019A1515110689]
- Foshan University High-level University Fund [20170131020]
- Foshan University Senior Talent Start Fund [20161110004]
This study demonstrated that ORFV strain NA1/11 can effectively inhibit the growth and migration of CRC cells both in vitro and in vivo, and regulates key factors relevant to apoptosis, autoimmunity/inflammation, angiogenesis, and the cell cycle. ORFV infection induces oncolytic activity by enhancing apoptosis, making it a potential therapeutic option for CRC.
Orf virus (ORFV) is a favorable oncolytic viral carrier in research, and ORFV strain NZ2 has been revealed to have antitumor effects in animal models mediated by immunoregulation profile. However, the antitumor effects triggered by the ORFV in colorectal cancer (CRC) cells is poorly characterized. The in vivo and in vitro roles of ORFV in CRC were determined using western blotting, colony formation, CCK-8, wound scratch assay, qPCR, and animal models. Furthermore, cytokine antibody chip assay, flow cytometry, western blotting, and immunohistochemical (IHC) assays were conducted to explore the potential mechanism of ORFV. The present data revealed that ORFV strain NA1/11 infected and inhibited the in vitro growth and migration of CRC cells. By establishing a CRC model in Balb/c mice, it was revealed that ORFV strain NA1/11 significantly inhibited the in vivo growth and migration of CRC cells. A cytokine antibody array was utilized to obtain a more comprehensive profile revealing the differentially expressed cytokines in ORFV infection. Cytokines, such as IL-7, IL-13, IL-15, CD27, CD30, pentraxin 3, and B lymphocyte chemoattractant (BLC), were upregulated. Axl, CXCL16, ANG-3, MMP10, IFN-gamma R1 and VEGF-B were downregulated. The results indicated that ORFV played roles in the regulation of key factors relevant to apoptosis, autoimmunity/inflammation, angiogenesis, and the cell cycle. Finally, data was presented to validate that ORFV infection induces oncolytic activity by enhancing apoptosis in vivo and in vitro. In conclusion, ORFV could be an oncolytic virus for CRC therapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据