期刊
NEW PHYTOLOGIST
卷 230, 期 2, 页码 867-877出版社
WILEY
DOI: 10.1111/nph.17165
关键词
BASL; BRXf; cell polarity; image quantification; stomatal lineage
资金
- German Research Foundation [438457603]
- National Science Foundation [1942722]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1942722] Funding Source: National Science Foundation
Pome is a versatile tool for quantifying cell polarity in various developmental contexts. Analysis in the Arabidopsis thaliana stomatal lineage reveals that the asynchrony and mutual dependency of polarity proteins are greater than previously thought. Analysis in the Brachypodium distachyon stomatal lineage shows that MAPKKK BdYDA1 is segregated and polarized following asymmetrical divisions.
Quantitative information on the spatiotemporal distribution of polarised proteins is central for understanding cell-fate determination, yet collecting sufficient data for statistical analysis is difficult to accomplish with manual measurements. Here we present Polarity Measurement (Pome), a semi-automated pipeline for the quantification of cell polarity and demonstrate its application to a variety of developmental contexts. Pome analysis reveals that, during asymmetric cell divisions in the Arabidopsis thaliana stomatal lineage, polarity proteins BASL and BRXL2 are more asynchronous and less mutually dependent than previously thought. A similar analysis of the linearly arrayed stomatal lineage of Brachypodium distachyon revealed that the MAPKKK BdYDA1 is segregated and polarised following asymmetrical divisions. Our results demonstrate that Pome is a versatile tool, which by itself or combined with tissue-level studies and advanced microscopy techniques can help to uncover new mechanisms of cell polarity.
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