期刊
NEUROCHEMISTRY INTERNATIONAL
卷 146, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2021.104972
关键词
Parkinson’ s disease; Pramipexole; miR-96; BNIP3; Mitophagy
资金
- Major Project of Department of Science and Technology of Hunan Province [2018SK1030]
Pramipexole alleviates neuronal injury by activating BNIP3-mediated mitophagy and reducing miR-96 in MPP+-induced cells.
Background: Parkinson?s disease is a common neurodegenerative problem. Pramipexole (PPX) plays protective role in Parkinson?s disease. Nevertheless, the mechanism of PPX in Parkinson?s disease-like neuronal injury is largely uncertain. Methods: 1-methyl-4-phenylpyridinium (MPP+)-stimulated neuronal cells and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mice were used as the model of Parkinson?s disease. MPP+-induced neuronal injury was assessed via cell viability, lactic dehydrogenase (LDH) release and apoptosis. microRNA-96 (miR-96) and BCL2/adenovirus E1B 19 kDa interacting protein 3 (BNIP3) abundances were examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or Western blotting. Mitophagy was tested by Western blotting and immunofluorescence staining. MPTP-induced neuronal injury in mice was investigated via behavioral tests and TUNEL. Results: PPX alleviated MPP+-induced neuronal injury via increasing cell viability and decreasing LDH release and apoptosis. PPX reversed MPP+-induced miR-96 expression and inhibition of mitophagy. miR-96 overexpression or BNIP3 interference weakened the suppressive role of PPX in MPP+-induced neuronal injury. miR96 targeted BNIP3 to inhibit PTEN-induced putative kinase 1 (PINK1)/Parkin signals-mediated mitophagy. miR96 overexpression promoted MPP+-induced neuronal injury via decreasing BNIP3. PPX weakened MPTP-induced neuronal injury in mice via regulating miR-96/BNIP3-mediated mitophagy. Conclusion: PPX mitigated neuronal injury in MPP+-induced cells and MPTP-induced mice by activating BNIP3mediated mitophagy via directly decreasing miR-96.
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