期刊
DNA REPAIR
卷 44, 期 -, 页码 169-177出版社
ELSEVIER
DOI: 10.1016/j.dnarep.2016.05.023
关键词
Ultraviolet; Cyclobutane pyrimidine dimer; Melanoma; Melanin; Chemiexcitation; Nitric oxide; Superoxide; Peroxynitrite; Triplet state
资金
- L'Oreal, Inc.
- NIH [5P50CA12197409]
Sunlight's ultraviolet wavelengths induce cyclobutane pyrimidine dimers (CPDs), which then cause mutations that lead to melanoma or to cancers of skin keratinocytes. In pigmented melanocytes, we found that CPDs arise both instantaneously and for hours after UV exposure ends. Remarkably, the CPDs arising in the dark originate by a novel pathway that resembles bioluminescence but does not end in light: First, UV activates the enzymes nitric oxide synthase (NOS) and NADPH oxidase (NOX), which generate the radicals nitric oxide (NO center dot) and superoxide (O-2(center dot-)); these combine to form the powerful oxidant peroxynitrite (ONOO-). A fragment of the skin pigment melanin is then oxidized, exciting an electron to an energy level so high that it is rarely seen in biology. This process of chemically exciting electrons, termed chemiexcitation, is used by fireflies to generate light but it had never been seen in mammalian cells. In melanocytes, the energy transfers radiationlessly to DNA, inducing CPDs. Chemiexcitation is a new source of genome instability, and it calls attention to endogenous mechanisms of genome maintenance that prevent electronic excitation or dissipate the energy of excited states. Chemiexcitation may also trigger pathogenesis in internal tissues because the same chemistry should arise wherever superoxide and nitric oxide arise near cells that contain melanin. (C) 2016 Elsevier B.V. All rights reserved.
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