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Identifying genome-wide off-target sites of CRISPR RNA-guided nucleases and deaminases with Digenome-seq

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NATURE PROTOCOLS
卷 16, 期 2, 页码 -

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NATURE RESEARCH
DOI: 10.1038/s41596-020-00453-6

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  1. Institute for Basic Science [IBS-R021-D1]
  2. KRIBB Research Initiative Program
  3. 'R&D Convergence Program' of the National Research Council of Science Technology [CAP-15-03-KRIBB]

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Digenome-seq is a cell-free method for identifying genome-wide off-target sites of programmable nucleases and deaminases, which is more sensitive and comprehensive than cell-based methods and does not involve DNA end enrichment through PCR amplification. The process takes about several weeks, including purification and preparation of RNPs, digestion of genomic DNA, and bioinformatic analysis after WGS.
Digested genome sequencing (Digenome-seq) is a highly sensitive, easy-to-carry-out, cell-free method for experimentally identifying genome-wide off-target sites of programmable nucleases and deaminases (also known as base editors). Genomic DNA is digested in vitro using clustered regularly interspaced short palindromic repeats ribonucleoproteins (RNPs; plus DNA-modifying enzymes to cleave both strands of DNA at sites containing deaminated base products, in the case of base editors) and subjected to whole-genome sequencing (WGS) with a typical sequencing depth of 30x. A web-based program is available to map in vitro cleavage sites corresponding to on- and off-target sites. Chromatin DNA, in parallel with histone-free genomic DNA, can also be used to account for the effects of chromatin structure on off-target nuclease activity. Digenome-seq is more sensitive and comprehensive than cell-based methods for identifying off-target sites. Unlike other cell-free methods, Digenome-seq does not involve enrichment of DNA ends through PCR amplification. The entire process other than WGS, which takes similar to 1-2 weeks, including purification and preparation of RNPs, digestion of genomic DNA and bioinformatic analysis after WGS, takes about several weeks.

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