期刊
NATURE MATERIALS
卷 20, 期 2, 页码 145-155出版社
NATURE PORTFOLIO
DOI: 10.1038/s41563-020-00804-4
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资金
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program [StG-2014-640525_REGMAMKID, CoG-2013-616480_TensionControl, CoG-2016-725722_OVOGROWTH, StG-757710]
- EFSD/Boehringer Ingelheim European Research Programme in Microvascular Complications of Diabetes
- EIT Health [20366]
- US National Science Foundation [CBET-0939511]
- Medical Research Council [MC_UP_1201/9]
- NIH [R01EB024591, R01EB025256]
- NSF [0939511, 1446474]
- European Commission [H2020-FETPROACT-01-2016-731957]
- Greenwall Foundation's 'Making a Difference' grant
- Spanish Ministry of Economy and Competitiveness (MINECO)/FEDER [SAF2015-72617-EXP, SAF2017-89782-R, PGC2018-099645-B-I00]
- Generalitat de Catalunya
- CERCA programme [2017 SGR 1306, SGR-2017-01602]
- Asociacion Espanola contra el Cancer (AECC) [LABAE16006]
- Instituto de Salud Carlos III (Tercel)
- Instituto de Salud Carlos III (Cardiocel)
- Instituto de Salud Carlos III (ACE2ORG)
- 'Centro de Excelencia Severo Ochoa' award from the MINECO (Agencia Estatal de Investigacion) [SEV2014-0425, CEX2018-000789-S]
- MIT-SPAIN la Caixa Foundation SEED FUND project Bioenginering Against Cancer - MISTI Global Seed Funds
- la Caixa Foundation
- SOLIDCAM ESTUDIO
- MRC [MC_UP_1201/9] Funding Source: UKRI
- Direct For Computer & Info Scie & Enginr
- Division Of Computer and Network Systems [1446474] Funding Source: National Science Foundation
Significant progress has been made in the differentiation of human pluripotent stem cells and the development of organoids. The convergence of stem cell biology and bioengineering allows for controlled stimuli to be provided to the system. Engineering solutions are emphasized to control the self-organization and functionality of stem cell-derived organoids.
In recent years considerable progress has been made in the development of faithful procedures for the differentiation of human pluripotent stem cells (hPSCs). An important step in this direction has also been the derivation of organoids. This technology generally relies on traditional three-dimensional culture techniques that exploit cell-autonomous self-organization responses of hPSCs with minimal control over the external inputs supplied to the system. The convergence of stem cell biology and bioengineering offers the possibility to provide these stimuli in a controlled fashion, resulting in the development of naturally inspired approaches to overcome major limitations of this nascent technology. Based on the current developments, we emphasize the achievements and ongoing challenges of bringing together hPSC organoid differentiation, bioengineering and ethics. This Review underlines the need for providing engineering solutions to gain control of self-organization and functionality of hPSC-derived organoids. We expect that this knowledge will guide the community to generate higher-grade hPSC-derived organoids for further applications in developmental biology, drug screening, disease modelling and personalized medicine. This Review provides an overview of bioengineering technologies that can be harnessed to facilitate the culture, self-organization and functionality of human pluripotent stem cell-derived organoids.
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