期刊
NANOTOXICOLOGY
卷 15, 期 3, 页码 352-365出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/17435390.2020.1863498
关键词
Fullerene C60; lung mechanics; alveolar collapse; mitochondrial function; ATP production; reactive oxygen species
资金
- Brazilian Council for Scientific and Technological Development (CNPq) [401372/2016-2, 302702/2017-2]
- Carlos Chagas Filho Rio de Janeiro State Research Supporting Foundation (FAPERJ) [E-26/202.785/2017]
In a study on male BALB/c mice, exposure to C60 fullerene nanoparticles led to impaired lung mechanics and mitochondrial function, increased reactive oxygen species concentration, and decreased ATP production.
C60 fullerene (C60) nanoparticles, a nanomaterial widely used in technology, can offer risks to humans, overcome biological barriers, and deposit onto the lungs. However, data on its putative pulmonary burden are scanty. Recently, the C60 interaction with mitochondria has been described in vitro and in vivo. We hypothesized that C60 impairs lung mechanics and mitochondrial function. Thirty-five male BALB/c mice were randomly divided into two groups intratracheally instilled with vehicle (0.9% NaCl + 1% Tween 80, CTRL) or C60 (1.0 mg/kg, FUL). Twenty-four hours after exposure, 15 FUL and 8 CTRL mice were anesthetized, paralyzed, and mechanically ventilated for the determination of lung mechanics. After euthanasia, the lungs were removed en bloc at end-expiration for histological processing. Lung tissue elastance and viscance were augmented in FUL group. Increased inflammatory cell number, alveolar collapse, septal thickening, and pulmonary edema were detected. In other six FUL and six CTRL mice, mitochondria expressed reduction in state 1 respiration [FUL = 3.0 +/- 1.14 vs. CTRL = 4.46 +/- 0.9 (SEM) nmol O-2/min/mg protein, p = 0.0210], ATP production (FUL = 122.6 +/- 18 vs. CTRL = 154.5 +/- 14 mu mol/100 mu g protein, p = 0.0340), and higher oxygen consumption in state 4 [FUL = 12.56 +/- 0.9 vs. CTRL = 8.26 +/- 0.6], generation of reactive oxygen species (FUL 733.1 +/- 169.32 vs. CTRL = 486.39 +/- 73.1 nmol/100 mu g protein, p = 0.0313) and reason ROS/ATP [FUL = 8.73 +/- 2.3 vs. CTRL = 2.99 +/- 0.3]. In conclusion, exposure to fullerene C60 impaired pulmonary mechanics and mitochondrial function, increased ROS concentration, and decrease ATP production.
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