期刊
NANO LETTERS
卷 21, 期 1, 页码 840-846出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c04600
关键词
fluorescence; super-resolution microscopy; DNA origami; single-molecule spectroscopy
类别
资金
- CONICET
- ANPCYT [PICT-2013-0792, PICT-2014-0739]
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy [EXC 2089/1-390776260, SFB1032]
- German Academic Exchange Service (DAAD)
- Boehringer Ingelheim Fonds
- German Academic Scholarship Foundation
p-MINFLUX is a new implementation of single-molecule localization method with simplified experimental setup and additional fluorescence lifetime information, delivering high photon efficiency. It demonstrates excellent performance in precise localization and lifetime imaging, applicable to both static and dynamic DNA Origami model systems.
We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to deliver the doughnut-shaped excitation foci at a maximum repetition rate. Using both static and dynamic DNA origami model systems, we demonstrate the performance of p-MINFLUX for single-molecule localization nanoscopy and tracking, respectively. p-MINFLUX delivers 1-2 nm localization precision with 2000-1000 photon counts. In addition, p-MINFLUX gives access to the fluorescence lifetime enabling multiplexing and super-resolved lifetime imaging. p-MINFLUX should help to unlock the full potential of innovative single-molecule localization schemes.
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