期刊
MYCORRHIZA
卷 31, 期 3, 页码 403-412出版社
SPRINGER
DOI: 10.1007/s00572-020-01016-z
关键词
Arbuscular mycorrhizal fungus; Hyphal exudate; Metabolite profiling; Phosphorus deficiency; Monoxenic culture
资金
- JSPS KAKENHI [JP15K07332, JP18K05368]
This study investigated the metabolite composition of extraradical hyphae of two AM fungal species under different P conditions. It was found that the concentrations of certain metabolites in the hyphal exudates were affected by P deficiency, with some metabolites increasing in concentration under low P conditions and others decreasing.
Arbuscular mycorrhizal (AM) fungal extraradical hyphae exude their metabolites into the soil. Root exudate metabolites are affected by plant species and P status. However, the effect of P status on AM hyphal exudate metabolites has been unknown. This study aimed to examine hyphal exudate metabolite composition of two AM fungal species and their response to P deficiency through metabolite profiling. Rhizophagus clarus and R. irregularis were grown in a two-compartment in vitro culture system of Linum usitatissimum roots on solid modified Strullu-Romand medium in combination with two P levels (3 mu M (P3) and 30 mu M (P30)). Hyphal exudates were collected from the hyphal compartment at 118 days after inoculation (DAI). The metabolite composition of the hyphal exudates was determined by capillary electrophoresis/time-of-flight mass spectrometry, resulting in the identification of a total of 141 metabolites at 118 DAI. In the hyphal exudates of R. clarus, the concentrations of 18 metabolites, including sugars, amino acids, and organic acids, were significantly higher (p < 0.05) under P3 than under P30 conditions. In contrast, the concentrations of 10 metabolites, including sugar and amino acids, in the hyphal exudates of R. irregularis were significantly lower (p < 0.05) under P3 than under P30 conditions. These findings suggest that the extraradical hyphae of AM fungi exude diverse metabolites of which concentrations are affected by P conditions and differ between AM fungal species.
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