期刊
MOLECULES
卷 26, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/molecules26020403
关键词
sunflower (Helianthus annuus L; ); flavonoids; response surface methodology; radical scavenging activities; chemical composition
资金
- Jilin University [SXGJSF2017-1]
- Jilin Province of China [SXGJSF2017-1]
- National Natural Science Foundation of China [31870201]
- Jilin Teyi Food Technology Co., Ltd.
This study focused on the extraction, radical scavenging activities, and chemical composition identification of total flavonoids in sunflower receptacles. The results showed that sunflower receptacles can be used as a natural source of flavonoids and have potential applications in the pharmaceutical industry.
This study was focused on extraction, radical scavenging activities, and chemical composition identification of total flavonoids in sunflower (Helianthus annuus L.) receptacles (TFSR). We investigated the optimal extract parameters of TFSR using response surface methodology. The highest yield of TFSR was 1.04% with the ethanol concentration 58%, the material-to-liquid ratio 1:20 (v/w), the extraction time 2.6 h, and the extraction temperature 67 degrees C. The results of radical scavenging activities showed that ethyl acetate fraction (EAF) was the strongest by using 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azino-bis (3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS) and iron ion reducing analysis. The EAF had the highest flavonoids contents. Four fractions A, B, C and D were enrichment from EAF by polyamide resin. Fraction B had the highest flavonoids content. Thirteen chemical components of flavonoids in fraction B were first identified by Ultimate 3000 Nano LC System coupled to a Q Exactive HF benchtop Orbitrap mass spectrometer (UHPLC-HRMS/MS). Among of the thirteen chemical components, isoquercetin and daidzein were identified accurately by comparing with standard samples. Radical scavenging analysis showed that isoquercetin and EAF had strong activities. Therefore, sunflower receptacles can be used as a source of natural flavonoids. TFSR as a natural radical scavenger has potential applications in pharmaceutical industry.
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