期刊
MOLECULES
卷 26, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/molecules26010215
关键词
antioxidant; Jakyakgamcho-tang; muscle atrophy; oxidative stress
资金
- Korea Institute of Oriental Medicine [KSN1911310, KSN2012330]
- National Research Council of Science & Technology (NST), Republic of Korea [KSN1911310] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ethanol extracts of JGT showed higher antioxidant activity and total phenolic and flavonoid contents compared to water extracts. JGT-E contained more active compounds and was more effective in protecting against oxidative stress-induced cell death, suggesting it could be a potential treatment for muscle aging and loss.
Oxidative stress is a major contributor to muscle aging and loss of muscle tissue. Jakyakgamcho-tang (JGT) has been used in traditional Eastern medicine to treat muscle pain. Here, we compared the total phenolic and flavonoid contents in 30% ethanol and water extracts of JGT and tested the preventive effects against oxidative stress (hydrogen peroxide)-induced cell death in murine C2C12 skeletal muscle cells. The total phenolic content and total flavonoid content in 30% ethanol extracts of JGT were higher than those of water extracts of JGT. Ethanol extracts of JGT (JGT-E) had stronger antioxidant activities of 2,2 '-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2 '-diphenyl-1-picrylhydrazyl-scavenging activity (DPPH) than water extracts of JGT (JGT-W). JGT-E contained 19-53% (1.8 to 4.9-fold) more active compounds (i.e., albiflorin, liquiritin, pentagalloylglucose, isoliquiritin apioside, isoliquiritin, liquiritigenin, and glycyrrhizin) than JGT-W. The ethanol extracts of JGT inhibited hydrogen peroxide-induced cell death and intracellular reactive oxygen species generation more effectively than the water extract of JGT in a dose-dependent manner. For the first time, these results suggest that ethanol extract of JGT is relatively more efficacious at protecting against oxidative stress-induced muscle cell death.
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