期刊
MOLECULAR NEUROBIOLOGY
卷 58, 期 4, 页码 1634-1649出版社
SPRINGER
DOI: 10.1007/s12035-020-02192-x
关键词
Extremely low-frequency magnetic field; SH-SY5Y; In vitro; 3D culture; MicroRNAs
资金
- Ente per le Nuove Tecnologie, l'Energia e l'Ambiente (ENEA-Italian National Agency for New Technologies Energy and Sustainable Economic Development) within the CRUI-CARE Agreement
The study characterized the response of SH-SY5Y cells to ELF-MF, showing that proliferation and apoptosis were not affected in both 2D and 3D cultures, but intracellular glutathione content and SOD1 expression were impaired in 3D cultures. ELF-MF combined with differentiation agents promoted neuroblastoma differentiation into a dopaminergic phenotype exclusively in 3D cultures, indicating the superiority of 3D culture as an experimental model for studying SH-SY5Y response to ELF-MF.
We here characterize the response to the extremely low-frequency (ELF) magnetic field (MF, 50 Hz, 1 mT) of SH-SY5Y human neuroblastoma cells, cultured in a three-dimensional (3D) Alvetex(R) scaffold compared to conventional two-dimensional (2D) monolayers. We proved that the growing phenotype of proliferating SH-SY5Y cells is not affected by the culturing conditions, as morphology, cell cycle distribution, proliferation/differentiation gene expression of 3D-cultures overlap what reported in 2D plates. In response to 72-h exposure to 50-Hz MF, we demonstrated that no proliferation change and apoptosis activation occur in both 2D and 3D cultures. Consistently, no modulation of Ki67, MYCN, CCDN1, and Nestin, of invasiveness and neo-angiogenesis-controlling genes (HIF-1 alpha, VEGF, and PDGF) and of microRNA epigenetic signature (miR-21-5p, miR-222-3p and miR-133b) is driven by ELF exposure. Conversely, intracellular glutathione content and SOD1 expression are exclusively impaired in 3D-culture cells in response to the MF, whereas no change of such redox modulators is observed in SH-SY5Y cells if grown on 2D monolayers. Moreover, ELF-MF synergizes with the differentiating agents to stimulate neuroblastoma differentiation into a dopaminergic (DA) phenotype in the 3D-scaffold culture only, as growth arrest and induction of p21, TH, DAT, and GAP43 are reported in ELF-exposed SH-SY5Y cells exclusively if grown on 3D scaffolds. As overall, our findings prove that 3D culture is a more reliable experimental model for studying SH-SY5Y response to ELF-MF if compared to 2D conventional monolayer, and put the bases for promoting 3D systems in future studies addressing the interaction between electromagnetic fields and biological systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据