期刊
MOLECULAR ECOLOGY
卷 30, 期 7, 页码 1594-1611出版社
WILEY
DOI: 10.1111/mec.15798
关键词
Aedes aegypti; endogenous viral elements; mosquito genomes; piRNA cluster
资金
- Human Frontier Science Program Research grant [RGP0007/2017]
- Italian Ministry of Education, University and Research FARE-MIUR project [R1623HZAH5]
- European Research Council Consolidator Grant (ERC-CoG) under the European Union's Horizon 2020 Programme [ERC-CoG 682394]
- European Research Council Consolidator Grant (ECR-CoG) under the European Union's Seventh Framework Programme [ERC-CoG 615680]
- VICI grant from the Netherlands Organization for Scientific Research (NWO) [016.VICI.170.090]
- Italian Ministry of Education, University and Research (MIUR): Dipartimenti Eccellenza Program (2018-2022)
Horizontal gene transfer from viruses to eukaryotic cells is a common phenomenon, with nrEVEs in Aedes aegypti potentially serving as a heritable, sequence-specific mechanism for antiviral immunity. The study shows that virus endogenization events are complex, with only a few nrEVEs playing a role in adaptive evolution in A. aegypti.
Horizontal gene transfer from viruses to eukaryotic cells is a pervasive phenomenon. Somatic viral integrations are linked to persistent viral infection whereas integrations into germline cells are maintained in host genomes by vertical transmission and may be co-opted for host functions. In the arboviral vector Aedes aegypti, an endogenous viral element from a nonretroviral RNA virus (nrEVE) was shown to produce PIWI-interacting RNAs (piRNAs) to limit infection with a cognate virus. Thus, nrEVEs may constitute a heritable, sequence-specific mechanism for antiviral immunity, analogous to piRNA-mediated silencing of transposable elements. Here, we combine population genomics and evolutionary approaches to analyse the genomic architecture of nrEVEs in A. aegypti. We conducted a genome-wide screen for adaptive nrEVEs and searched for novel population-specific nrEVEs in the genomes of 80 individual wild-caught mosquitoes from five geographical populations. We show a dynamic landscape of nrEVEs in mosquito genomes and identified five novel nrEVEs derived from two currently circulating viruses, providing evidence of the environmental-dependent modification of a piRNA cluster. Overall, our results show that virus endogenization events are complex with only a few nrEVEs contributing to adaptive evolution in A. aegypti.
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