期刊
MOLECULAR CANCER
卷 20, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12943-021-01314-w
关键词
circSDHC; miR-127-3p; Renal cell carcinoma
资金
- Natural Science Foundation of China [81772514, 82073381, 81772718, 81725016, 81902576, 81872094]
- Pearl River S&T Nova Program of Guangzhou [201806010005, 2018A030310327]
- Natural Science Foundation of Guangdong [S2019B151502046]
- Medical Scientific Research Foundation of Guangdong Province [A2018051]
- Guangdong Provincial Science and Technology Foundation of China [2017B020227004]
CircSDHC expression is correlated with advanced TNM stage and poor survival in RCC patients, and it promotes RCC progression by competitively binding to miR-127-3p, thereby preventing its suppression of CDKN3 and the E2F1 pathway. This suggests that circSDHC could be a potential therapeutic target in RCC treatment.
Background There is increasing evidence that circular RNAs (circRNAs) have significant regulatory roles in cancer development and progression; however, the expression patterns and biological functions of circRNAs in renal cell carcinoma (RCC) remain largely elusive. Method Bioinformatics methods were applied to screen for circRNAs differentially expressed in RCC. Analysis of online circRNAs microarray datasets and our own patient cohort indicated that circSDHC (hsa_circ_0015004) had a potential oncogenic role in RCC. Subsequently, circSDHC expression was measured in RCC tissues and cell lines by qPCR assay, and the prognostic value of circSDHC evaluated. Further, a series of functional in vitro and in vivo experiments were conducted to assess the effects of circSDHC on RCC proliferation and metastasis. RNA pull-down assay, luciferase reporter and fluorescent in situ hybridization assays were used to confirm the interactions between circSDHC, miR-127-3p and its target genes. Results Clinically, high circSDHC expression was correlated with advanced TNM stage and poor survival in patients with RCC. Further, circSDHC promoted tumor cell proliferation and invasion, both in vivo and in vitro. Analysis of the mechanism underlying the effects of circSDHC in RCC demonstrated that it binds competitively to miR-127-3p and prevents its suppression of a downstream gene, CDKN3, and the E2F1 pathway, thereby leading to RCC malignant progression. Furthermore, knockdown of circSDHC caused decreased CDKN3 expression and E2F1 pathway inhibition, which could be rescued by treatment with an miR-127-3p inhibitor. Conclusion Our data indicates, for the first time, an essential role for the circSDHC/miR-127-3p/CDKN3/E2F1 axis in RCC progression. Thus, circSDHC has potential to be a new therapeutic target in patients with RCC.
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