4.4 Article

Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis

期刊

MOLECULAR BIOLOGY OF THE CELL
卷 32, 期 5, 页码 391-401

出版社

AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E20-11-0745

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资金

  1. National Institutes of Health/National Aging Institute [R21AG066038]
  2. Clinical and Translational Science Institute, Medical College of Wisconsin [TL1TR001437]
  3. Foundation Fighting Blindness [PPA-0617-0718]

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The study investigated the genetic interactions of ctage5 and tango1 in zebrafish, revealing their diverse roles in organogenesis, collagen versus lipoprotein trafficking, stress-pathway activation, and survival. Although Ctage5 and Tango1 have overlapping functions in tissue development and homeostasis, they also exhibit divergent roles in these processes.
DCoat protein complex II (COPII) factors mediate cargo export from the endoplasmic reticulum (ER), but bulky collagens and lipoproteins are too large for traditional COPII vesicles. Mammalian CTAGE5 and TANGO1 have been well characterized individually as specialized cargo receptors at the ER that function with COPII coats to facilitate trafficking of bulky cargoes. Here, we present a genetic interaction study in zebrafish of deletions in ctage5, tango1, or both to investigate their distinct and complementary potential functions. We found that Ctage5 and Tango1 have different roles related to organogenesis, collagen versus lipoprotein trafficking, stress-pathway activation, and survival. While disruption of both ctage5 and tango1 compounded phenotype severity, mutation of either factor alone revealed novel tissue-specific defects in the building of heart, muscle, lens, and intestine, in addition to previously described roles in the development of neural and cartilage tissues. Together, our results demonstrate that Ctage5 and Tango1 have overlapping functions, but also suggest divergent roles in tissue development and homeostasis.

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