期刊
MOLECULAR AND CELLULAR NEUROSCIENCE
卷 110, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2020.103573
关键词
Pre-conditioning response; In vitro priming; DRG; Satellite glial cells; Neurite outgrowth; Nerve regeneration
资金
- Canadian Institutes of Health Research (CIHR) [FRN148675]
- College of Medicine, University of Saskatchewan
This study introduces a novel rapid screening model for nerve regeneration therapeutics, focusing on adult neurons. The model incubates sensory ganglia for 24 hours prior to dissociation, revealing unique events that promote axon growth. It provides a platform for rapid screening of drug candidates and offers opportunities to study novel intrinsic molecular changes in neurons and glial cells aimed at improving the pace of axon growth.
Peripheral nerve injuries have the potential to bring about long-term disabilities in individuals. The major issue in repairing nerve injuries is the poor growth rate of axons. Although several molecules have been identified as potential candidates for improving axon growth, their potential translation into clinical practice is preliminary and largely unexplored. This necessitates identifying additional molecular candidates with superior potential to improve axon growth. Lack of a simple non-surgical screening model also poses a hurdle in rapidly screening potential candidate molecules. In this work, we developed a novel, rapid screening model for nerve regeneration therapeutics that retains a focus on adult neurons. The model involves simple incubation of sensory ganglia over a period of 24 h prior to dissociation. Surprisingly, this model features unique events that reprogram both sensory neurons and supporting glia favoring axon growth. Moreover, several associated cellular and molecular changes involved in this model partially mimic classic axotomy-induced changes in sensory ganglia. Overall, this model presents with a platform that not only allows rapid screening of drug candidates but offers opportunities in studying novel intrinsic molecular changes in both neurons and glial cells directed towards improving the pace of axon growth.
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