期刊
MICROCHEMICAL JOURNAL
卷 160, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2020.105703
关键词
Fluorescent probes; Sulfur dioxide; Near-infrared; Cell imaging; In vivo imaging
资金
- National Natural Science Foundation of China [21961010, 21804102]
- Health and Family Planning Research Project of Hainan [18A200073]
- Talent Program of Hainan Medical University [XRC190034]
- Outstanding Young and Middle-aged Scientific Innovation Team of Colleges and Universities of Hubei Province: Biomass chemical technologies and materials [T201908]
- Hainan High-Level Talents Program [HNRCQ201903724]
The study reports a ratiometric NIR fluorescent probe Cou-Rh for monitoring bisulfite based on a Michael addition reaction, which demonstrates high sensitivity and selectivity in detecting bisulfite in living cells and zebrafish larvae.
Sulfur dioxide (SO2), a possible endogenous gaseous signaling molecule, has been found to be correlated with various diseases in living organisms. Thus, the development of effective methods for detection of SO2 are of great physiological and pathological significance. In this work, we report a ratiometric NIR fluorescent probe Cou-Rh for monitoring bisulfite on basis of Michael addition reaction. This probe is prepared from coumarin by acid -catalyzed cyclization reaction. The addition of bisulfite results in a significant decrease of fluorescence intensity at 695 nm and enhancement at 550 nm. The probe also displays high selectivity over various biologically related analytes with nanomolar limit of detection. What's more, fluorescence imaging further confirms that the probe is capable of detecting bisulfite in living cells and zebrafish larvae. The results demonstrate that probe CouRh can be employed for a useful scaffold to study the physiological function of sulfur dioxide.
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