Augmented spectrofluorimetric determination of certain fluoroquinolones via micellar - Metal complex connection: Application to pharmaceuticals and biological fluids

Simple, sensitive, rapid and specific spectroftuorimetric procedure has been described for the analysis of fluoroquinolones namely Ofloxacin (OFX), Levofloxacin (LVX) and Pefloxacin (PFX) in Pharmaceuticals and biological fluids. The named drugs reacted with Al+3 to produce highly fluorescent stable complexes measured at lambda(em) ranged from 436 to 480 nm (lambda(ex) 329-333 nm). The fluorescence intensity of the produced complex could be enhanced by the addition of sodium dodecyl sulfate. The method had a good linearity within the concentrations ranged from 8.0 to 140.0 ng mL(-1) for the cited drugs. The detection limits of the proposed method were 2.6, 3.2 and 4.50 ng mL(-1) for the studied drugs. Factors affecting the fluorescence intensity of the resultant chelates were cautiously examined and adjusted. The developed method was employed effectively on the analysis of commercially available dosage forms with excellent accuracy and precision. Furthermore, the method was capable for the in-vitro as well as in vivo estimation of the investigated drugs in human plasma. The luminescence mechanism of the system is also discussed in detail, and the stoichiometry of the chelate formation reaction was obtained by Job's method.

Automated summary

A simple, sensitive, rapid, and specific spectroftuorimetric procedure was developed for the analysis of fluoroquinolones in Pharmaceuticals and biological fluids. The method showed good linearity, low detection limits, and excellent accuracy for the determination of different drugs.