期刊
MICROCHEMICAL JOURNAL
卷 160, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2020.105683
关键词
Androgens; Monoclonal antibody; Indirect competitive enzyme-linked immunosorbent assay; Animal edible tissues
资金
- National Natural Science Foundation of China [31772074]
- Fundamental Research Funds for the Central Universities [2662017PY049]
A broad-spectrum monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for rapidly screening androgens in various animal tissues. The method showed good correlation with HPLC-MS/MS results, demonstrating its effectiveness and accuracy for detecting androgens in animal tissues.
Androgens efficiently promote skeletal muscle growth and protein synthesis but causes neurotoxicity, genotoxicity and carcinogenicity, which had been prohibited applying in livestock. In this study, a broad-spectrum monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed for rapidly screening of androgens in various animal tissues. Primarily, a group-specific mAb, 4D12, was produced and showed IC50 values for nortestosterone, methyltestosterone, testosterone and trenbolone of 2.2, 0.3, 1.6 and 4.4 mu g L-1, respectively. The limits of detection and limits of quantification of this method for 4 androgens in 11 kinds of animal edible tissues ranged from 37.2 to 697.8 ng L-1 and 70.0 to 1524.0 ng L-1, respectively. The recoveries of the 11 kinds of samples spiked with 4 androgens were in the range from 65.0% to 106.6%, with coefficients of variation below 16.9%. A good correlation (R-2 = 0.998) was shown between the ic-ELISA and HPLC-MS/MS results for beef.
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