Safranin amplified carbon paste electrode sensor for analysis of paracetamol and epinephrine in presence of folic acid and ascorbic acid

Here in the present study, safranin (SAF) amplified electrochemical sensor was designed for the individual and simultaneous quantification of Paracetamol (PA), Epinephrine (EP), Folic acid (FA) and Ascorbic acid (AA). The construction of modified electrode was carried by electropolymerization of SAF on the surface of bare carbon paste electrode (BCPE). The electrochemical performances were studied by cyclic voltammetry (CV), linear sweep voltammetry (LSV) and differential pulse voltammetry (DPV) at designed electrode. The superficial morphology of bare and modified carbon paste electrode (MCPE) was characterized by Scanning electron microscope (SEM). The developed electrode depicts the very good interaction with these bio-molecules and shows fine sensitivity and selectivity. The designed electrode process was found to be both adsorption and diffusion controlled phenomena and the lower limit detection (LOD) for PA and EP got at 0.47 x 10(-6) and 0.61 x 10(-6) M respectively. As well, the developed electrode was applied for the practical sample analysis for PA and EP with agreeable results and it consistent in-between 96.84 and 100.2%.

Automated summary

In this study, a SAF amplified electrochemical sensor was developed for the quantification of multiple biomolecules, showing good sensitivity and selectivity. The modified electrode, created by electropolymerization of SAF on BCPE, exhibited adsorption and diffusion controlled phenomena. Practical sample analysis using the developed electrode yielded satisfactory results with high consistency.