期刊
MICROBIAL PATHOGENESIS
卷 149, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2020.104349
关键词
Scedosporium apiospermum; Neutrophil extracellular traps; Pulmonary infection murine model; Elastase; Myeloperoxidase
资金
- Consejo Nacional de Ciencia y Tecnologia [CONACyT-INFRA-2015-01-251142]
- Programa de Apoyo a la Investigacion Cientifica y Tecnologica PAICYT [SA-1445-20]
Scedosporium apiospermum is an opportunistic emerging pathogen that can develop in both immunosuppressive and immunocompetent patients with pulmonary infections. Neutrophils are recognized as critical cells in the early response to a fungal infection through different mechanisms that eliminate or control the infection such as neutrophil extracellular traps (NETs). In this work, we investigate the presence of NETs in the lung tissue of immunocompetent mice infected with Scedosporium apiospermum. In the histopathological study the presence of filamentous basophilic material with hematoxylin-eosin and periodic acid Schiff stains suggestive of extra cellular DNA was observed. We demonstrated the presence of NETs by immunofluorescence staining of extra cellular DNA, myeloperoxidase, and elastase in lung tissue. Our results showed that on days 1 and 3 post infection extracellular DNA, myeloperoxidase, and elastase correlate with areas of high concentration of cell infiltrates and fungal structures. The observation of fungal structures in the tissue decreased as did the presence of NETs by day 5 post-infection. We suggest that NETs release may play an important role in the early containment of Scedosporium apiospermum lung infection.
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