期刊
MICROBIAL PATHOGENESIS
卷 149, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2020.104503
关键词
Vibrio splendidus; Flagellin; Overexpression of fliC; Adhesion
资金
- Zhejiang Provincial Natural Science Foundation [LZ19C190001]
- National Natural Science Foundation of China [41676141, 31972833]
- Natural Science Foundation for Distinguished Young Scholar of Zhejiang Province, China [LR20C190001]
- Natural Science Foundation of Ningbo City [2019A610418]
- Ningbo Public Welfare Project [2019C10074]
- K.C. Wong Magna Fund at Ningbo University
Vibrio splendidus-related strains are important opportunistic marine pathogens, and they can infect many important marine animals, including the sea cucumber Apostichopus japonicus. In this study, one gene coding flagellin was cloned and a V. splendidus-related strain AJ01/GFPFliC with the overexpression of fliC gene was constructed to explore the function of FliC. AJ01/GFPFliC showed a 3-4 h delay in the initial growth phase and then its growth was faster than that of the wild type strain AJ01. The abilities of swarming motility and biofilm formation ability of AJ01/GFPFliC were also higher than that of AJ01. The adhesion rate of AJ01/GFPFliC to the slide and the coelomocytes of A. japonicus increased from 1% to 5%, and 25% to 40%, respectively, and the adhered AJ01/GFPFliC cells in intestinal tissue of A. japonicus reached 8.0 x 10(6) CFU/g, which was 2.5-fold higher than that of the control strain AJ01/GFP. Concluded from all the data suggested that FliC was an adhesion factor of V. splendidus-related strain AJ01 that could also contribute to bacterial swarming motility and biofilm formation.
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