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DOI: 10.1016/j.msec.2021.111867
关键词
Silk fibroin scaffold; Weft knitting; Mechanical properties; Biological properties; Tissue engineering; Cell culture
3D scaffolds are crucial for tissue engineering, and this study shows how different fabrication techniques using the same biomaterial can lead to varied characteristics in the scaffolds. Among the techniques, weft knitting showed outstanding cell-scaffold interaction and mechanical strength, making it suitable for load-bearing soft tissue regeneration like the urinary bladder.
3D scaffolds are in the center of attention for tissue engineering applications. Whilst many studies have focused on the biological properties of scaffolds, less attention has been paid to meeting the biomechanics of the target tissues. In this work, we show how using the same original biomaterial, but different fabrication techniques can lead to a broad range of structural, mechanical, and biological characteristics. Starting with silk fibroin filament as our base biomaterial, we employed electrospinning, film casting, and weft knitting as different scaffold fabrication techniques. Among these three, the weft knit scaffold showed outstanding cell-scaffold interaction including full 3D cell attachment, complete cell coverage around individual filaments, and in-depth cell infiltration. Post-fabrication degumming of silk filament yarns resulted in more bulky and less open pores for the silk fibroin knit scaffold. The decreased pore size after degumming of knit scaffold alleviated the need to in-advance pore filling (a requisite for increasing cell adhesion in a typical knit scaffold having big pores). From a mechanical viewpoint, the weft knit scaffold shows the highest mechanical strength alongside with far better extensibility. Interestingly, the silk filament weft knit scaffold (in the course direction) was 100 and 1000 times more compliant than silk fibroin film and electrospun web, respectively. The observed effect of material type and fabrication technique highlights the suitability of silk fibroin weft-knit scaffolds for the regeneration of load bearing soft tissues such as urine bladder.
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