期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2020.111476
关键词
Tissue engineering; Magnetic nanoparticles; Biomaterials; Bio-distribution; In vivo biocompatibility
资金
- Instituto de Salud Carlos III -ISCIII (Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica I + D + i from the Spanish Ministerio de Ciencia e Innovacion) [FIS-PI17/0391, FIS-PI17/0393]
- ERDF-FEDER, European Union
- ISCIII thorough AES 2017 [AC17/00013]
- EuroNanoMed
- Ministerio de Economia, Industria y Competitividad, MINECO [FIS2017-85954-R]
- Agencia Estatal de Investigacion, AEI, Spain
- Fondo Europeo de Desarrollo Regional, FEDER, European Union
- Consejeria de Salud y Familias, Junta de Andalucia, Spain [CS PI-0257-2017, CSyF PE-0395-2019]
- SECYT (Secretary of Science and Technology of National University of Cordoba, Argentina) [Res SECYT 411/18]
- Project Future Investments UCA JEDI, project RheoGel by the French Agence Nationale de la Recherche [ANR-15-IDEX-01]
Artificial tissues with potential for local therapy have been created using magnetic-responsive nanoparticles (MNPs) in tissue engineering. In this study, magnetic fibrin-agarose tissue-like biomaterials were characterized in vivo, showing presence of MNPs in the graft area after 12 weeks. The study demonstrated improved biomechanical properties, biosafety, and biocompatibility for potential clinical use of these biomaterials as an alternative delivery route for magnetic nanoparticles.
Novel artificial tissues with potential usefulness in local-based therapies have been generated by tissue engineering using magnetic-responsive nanoparticles (MNPs). In this study, we performed a comprehensive in vivo characterization of bioengineered magnetic fibrin-agarose tissue-like biomaterials. First, in vitro analyses were performed and the cytocompatibility of MNPs was demonstrated. Then, bioartificial tissues were generated and subcutaneously implanted in Wistar rats and their biodistribution, biocompatibility and functionality were analysed at the morphological, histological, haematological and biochemical levels as compared to injected MNPs. Magnetic Resonance Image (MRI), histology and magnetometry confirmed the presence of MNPs restricted to the grafting area after 12 weeks. Histologically, we found a local initial inflammatory response that decreased with time. Structural, ultrastructural, haematological and biochemical analyses of vital organs showed absence of damage or failure. This study demonstrated that the novel magnetic tissue-like biomaterials with improved biomechanical properties fulfil the biosafety and biocompatibility requirements for future clinical use and support the use of these biomaterials as an alternative delivery route for magnetic nanoparticles.
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