期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2020.111407
关键词
Protein A; Myelination; Electrospinning; Neurons; Oligodendrocytes; Nerve regeneration
资金
- A*Star BMRC International Joint Grant -Singapore-China Joint Research Program [1610500024]
- Singapore National Research Foundation under its NMRCCBRG grant [NMRC/CBRG/0096/2015]
- Ministry of Education (Singapore) [RG38/19]
- SingHealth-NTU-Research Collaborative Grant [SHS-NTU/038/206]
This study presents a Protein A-based biomimicking platform that efficiently orients proteins for evaluating cellular behavior, demonstrating efficacy in neural cell models. The results suggest the platform's potential for protein screening applications.
A proper protein orientation is often required in order to achieve specific protein-receptor interaction to elicit a desired biological response. Here, we present a Protein A-based biomimicking platform that is capable of efficiently orienting proteins for evaluating cellular behaviour. By absorbing Protein A onto aligned bio-mimicking polycaprolactone (PCL) fibers, we demonstrate that protein binding could be retained on these fibers for at least 7 days under physiologically relevant conditions. We further show that Protein A served as a molecular orientor to arrange the recombinant proteins in similar orientations. Such protein-orienting scaffolds were further verified to be biologically functional by using sensitive primary rat cortical neurons (CNs) and oligodendrocyte progenitor cells (OPCs), as model neural cells for a stringent proof of concept. Specifically, CNs that were seeded on fibers coated with Protein A and a known enhancer of neurite growth (L1 Cell Adhesion Molecular L1CAM) displayed the longest total neurite length (462.77 +/- 100.79 mu m, p < 0.001) as compared to the controls. Besides that, OPCs seeded on fibers coated with Protein A and Neuregulin-1 Type III (Nrg1 type III) (myelin enhancer) produced the longest myelin ensheathment length (19.8 +/- 11.69 mu m). These results demonstrate the efficacy of this platform for protein screening applications.
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