期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2020.111440
关键词
Multilayer nanofilm; Laponite (R); Bone extracellular matrix; Layer-by-layer; Osteogenic differentiation
资金
- UK-Korea Partnering Award - UK Medical Research Council (MRC)
- Korea Health Industry Development Institute (KHIDI) [MC_PC_18015]
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/L010259/1]
- UK Regenerative Medicine Platform Acellular / Smart Materials - 3D Architecture [MR/R015651/1]
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI18C2021]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [NRF-2017R1E1A1A01074343]
- EPSRC [EP/L010259/1] Funding Source: UKRI
- MRC [MR/R015651/1] Funding Source: UKRI
A novel ECM/LAP nanofilm layer material was fabricated with potential application in hard tissue engineering, by enhancing mechanical stability and osteogenic differentiation potential.
Functionalized scaffolds hold promise for stem cell therapy by controlling stem cell fate and differentiation potential. Here, we have examined the potential of a 2-dimensional (2D) scaffold to stimulate bone regeneration. Solubilized extracellular matrix (ECM) from human bone tissue contains native extracellular cues for human skeletal cells that facilitate osteogenic differentiation. However, human bone ECM displays limited mechanical strength and degradation stability under physiological conditions, necessitating modification of the physical properties of ECM before it can be considered for tissue engineering applications. To increase the mechanical stability of ECM, we explored the potential of synthetic Laponite (R) (LAP) clay as a counter material to prepare a 2D scaffold using Layer-by-Layer (LbL) self-assembly. The LAP and ECM multilayer nanofilms (ECM/LAP film) were successfully generated through electrostatic and protein-clay interactions. Furthermore, to enhance the mechanical properties of the ECM/LAP film, application of a NaCl solution wash step, instead of deionized water following LAP deposition resulted in the generation of stable, multi-stacked LAP layers which displayed enhanced mechanical properties able to sustain human skeletal progenitor cell growth. The ECM/LAP films were not cytotoxic and, critically, showed enhanced osteogenic differentiation potential as a consequence of the synergistic effects of ECM and LAP. In summary, we demonstrate the fabrication of a novel ECM/LAP nanofilm layer material with potential application in hard tissue engineering.
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