期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2020.111454
关键词
Diamond electrode; Laminin coating; Biocompatibility; Neural stimulation; Charge injection capacity
资金
- CASS Foundation
- National Health and Medical Research Council (NHMRC) of Australia [GNT1101717]
- Australian Research Council (ARC) [DP120103405]
Coating electrodes with biomolecules like extracellular matrix proteins can suppress adverse biological responses and maintain optimal electrochemical performance for neural stimulation. The method of covalently coupling biomolecules to diamond electrodes enhances cell attachment densities and neurite outgrowth, showing promise in reducing inflammatory responses in vivo.
The performance of many implantable neural stimulation devices is degraded due to the loss of neurons around the electrodes by the body's natural biological responses to a foreign material. Coating of electrodes with biomolecules such as extracellular matrix proteins is one potential route to suppress the adverse responses that lead to loss of implant functionality. Concurrently, however, the electrochemical performance of the stimulating electrode must remain optimal to continue to safely provide sufficient charge for neural stimulation. We have previously found that oxygen plasma treated nitrogen included ultrananocrystalline diamond coated platinum electrodes exhibit superior charge injection capacity and electrochemical stability for neural stimulation (Sikder et al., 2019). To fabricate bioactive diamond electrodes, in this work, laminin, an extracellular matrix protein known to be involved in inter-neuron adhesion and recognition, was used as an example biomolecule. Here, laminin was covalently coupled to diamond electrodes. Electrochemical analysis found that the covalently coupled films were robust and resulted in minimal change to the charge injection capacity of diamond electrodes. The successful binding of laminin and its biological activity was further confirmed using primary rat cortical neuron cultures, and the coated electrodes showed enhanced cell attachment densities and neurite outgrowth. The method proposed in this work is versatile and adaptable to many other biomolecules for producing bioactive diamond electrodes, which are expected to show reduced the inflammatory responses in vivo.
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