4.5 Article

Seasonal monitoring of lipid degradation processes in the western English Channel links bacterial 10S-DOX enzyme activity to free fatty acid production by phytoplankton

期刊

MARINE CHEMISTRY
卷 230, 期 -, 页码 -

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ELSEVIER
DOI: 10.1016/j.marchem.2021.103928

关键词

Biotic and abiotic degradation; 10S-DOX enzymatic activity; Bacteria; Wounded diatoms

资金

  1. Centre National de la Recherche Scientifique (CNRS)
  2. Aix-Marseille University
  3. FEDER OCEANOMED [1166-39417]
  4. UK Natural Environment Research Council's National Capability Long-term Single Centre Science Programme, Climate Linked Atlantic Sector Science [NE/R015953/1]

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Recent studies have observed the action of a bacterial dioxygenase on palmitoleic acid in polar and estuarine settings. In order to further understand the enzyme's mechanism in marine environments, lipid distributions and degradation products were analyzed in water samples collected in 2018. The research showed varying lipid degradation pathways and activities in different seasons, with bacterial 10S-DOX playing a significant role during the spring bloom.
In a few recent studies, the action of a bacterial dioxygenase (10S-DOX) on palmitoleic acid was observed within some polar and estuarine settings. To add further mechanistic information regarding the action of this enzyme in marine settings, we measured a range of lipids (sterols, fatty acids and the chlorophyll phytyl side chain) and their biotic and abiotic degradation products in water samples collected in 2018 from two depths (5 m and 25 m) at the temperate oceanographic time series site L4, located in the western English Channel. Lipid distributions indicated a dominance of diatoms and copepods during the spring bloom, while a peak in dinoflagellate activity was evident in samples collected from late summer/autumn, both outcomes being consistent with taxonomic data reported previously for the same sampling site and interval. Monitoring of lipid oxidation products characteristic of different degradation pathways showed a relatively weak effect of photo- and autoxidation processes, with these acting mainly on the more reactive lipids (i.e. chlorophyll and polyunsaturated fatty acids). In contrast, monitoring of biotic degradation processes revealed significant quantities of 10S-hydroxyhexadec-8(E)enoic acid in samples collected at the end of April (reaching 40% of the residual parent palmitoleic acid), attributed to the involvement of bacterial 10-dioxygenase (10S-DOX) activity during the spring bloom. We propose that this enzyme could be utilised by bacteria to detoxify free fatty acids released by wounded diatoms in the presence of copepods

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