期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 135, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2020.110249
关键词
Staphylococcus aureus; Improved propidium monoazide (PMAxx); Viable bacteria detection; Recombinase aided amplification; Artificially contaminated milk
资金
- China National Key RD Program [2016YFF0203204]
- Natural Science Foundation of Zhejiang Province, China [LY16H260004]
- Research Foundation from Academic and Technical Leaders of Major Disciplines in Jiangxi Province, China [20194BCJ22004]
- Research Foundation from State Key Laboratory of Food Science and Technology, Nanchang University, China [SKLF-ZZA-201912]
The study proposed a method for rapid and real-time detection of viable S. aureus in milk, combining PMAxx dye and RAA technology. It can accurately detect S. aureus in a short time, which is of great significance for effective monitoring of food safety.
Staphylococcus aureus is a common foodborne pathogenic microorganism. A modified propidium monoazide (PMAxx) dye combined with recombinase aided amplification (RAA) was proposed for the rapid and real-time detection of viable S. aureus. PMAxx, a kind of nucleic acid dye is used to distinguish viable bacteria from dead bacteria. Additionally, RAA technique has the advantage of a short detection time (20 min) and uniform temperature (39 degrees C), which is of great significance for the efficient detection of S. aureus. The S. aureus nuc gene was used as primer and probe in a microplate assay. The effect of PMAxx treatment exhibited permeation of the dead cell membranes that was observed under confocal laser scanning microscope (CLSM). After the enrichment of viable target bacteria ranging from 5.4 x 10(1) to 5.4 x 10(3) CFU/mL, the LOD for viable S. aureus was 10(2) CFU/mL under 3 h enrichment and 10(1) CFU/mL under 6 h enrichment in artificially contaminated milk, respectively. In the presence of nontarget bacteria at 10(6) CFU/mL, the system could detect 10(7) CFU/mL S. aureus in milk sample. The proposed PMAxx-RAA assay exhibited potential for the accurate and rapid viable S. aureus detection in milk.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据