期刊
LIMNOLOGY AND OCEANOGRAPHY-METHODS
卷 19, 期 2, 页码 145-157出版社
WILEY
DOI: 10.1002/lom3.10412
关键词
-
资金
- German Research Foundation [CRC 1127]
- European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant [642575]
- ProjektDEAL
N-acyl homoserine lactones (AHLs) are signaling molecules produced by Gram-negative bacteria for cell-cell communication, influencing marine bacterial processes and interactions with microalgae. Measuring AHL concentrations is challenging but important for ecological research and biofilm control. A novel sampling and analysis method has been developed to detect and quantify AHLs in marine sediment samples efficiently.
N-acyl homoserine lactones (AHLs) are molecules produced by many Gram-negative bacteria as mediators of cell-cell signaling in a mechanism known as quorum sensing (QS). QS is widespread in marine bacteria regulating diverse processes, such as virulence or excretion of polymers that mediate biofilm formation. Associated eukaryotes, such as microalgae, respond to these cues as well, leading to an intricate signaling network. To date, only very few studies attempted to measure AHL concentrations in phototrophic microbial communities, which are hot spots for bacteria-bacteria as well as microalgae-bacteria interactions. AHL quantification in environmental samples is challenging and requires a robust and reproducible sampling strategy. However, knowing about AHL concentrations opens up multiple perspectives from answering fundamental ecological questions to deriving guidelines for manipulation and control of biofilms. Here, we present a method for sampling and AHL identification and quantification from marine intertidal sediments. The use of contact cores for sediment sampling ensures reproducible sample surface area and volume at each location. Flash-freezing of the samples with liquid nitrogen prevents enzymatic AHL degradation between sampling and extraction. After solvent extraction, samples were analyzed with an ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) method that allows to baseline-separate 16 different AHLs in less than 10 min. The sensitivity of the method is sufficient for detection and quantification of AHLs in environmental samples of less than 16 cm(3).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据