期刊
LIFE SCIENCES
卷 262, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2020.118548
关键词
High glucose; Diabetes mellitus; Nuclear factor-kappa B; Interleukin-10; Transcriptomics; Cholangiocarcinoma; Biliary tract cancer; STAT3
资金
- Prince Mahidol Award Youth Program of Prince Mahidol Award Foundation under the Royal Patronage of HM the king of Thailand
- Khon Kaen University [601802, I62-01-02]
- NIHGRI [U54 HG003273]
Aims: The present report aimed to investigate the underlying genes and pathways of high glucose driving cholangiocarcinoma (CCA) aggressiveness. Main methods: We screened and compared the gene expression profiles obtained by RNA sequencing, of CCA cells cultured in high and normal glucose. Results from the transcriptomic analysis were confirmed in additional cell lines using in vitro migration-invasion assay, Western blotting and immunocytofluorescence. Key findings: Data indicated that high glucose increased the expression of interleukin-10 (IL-10), an upstream regulator of nuclear factor-kappa B (NF-kappa B) pathway, through the nuclear localization of NF-kappa B. High glucose-induced NF-kappa B increased the migration and invasion of CCA cells and the expression of downstream NF-kappa B targeted genes associated with aggressiveness, including interleukin-6, a potent triggering signal of the signal transducer and activator of transcription 3 (STAT3) pathway. Such effects were reversed by inhibiting NF-kappa B nuclear translocation which additionally reduced the phosphorylation of STAT3 at Y705. Significance: These results indicate that NF-kappa B is activated by high glucose and they suggest that NF-kappa B interaction with STAT3 enhances CCA aggressiveness. Therefore, targeting multiple pathways such as STAT3 and NF kappa B might improve CCA treatment outcome especially in condition such as hyperglycemia.
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