期刊
LANGMUIR
卷 37, 期 4, 页码 1542-1550出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.0c03159
关键词
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资金
- Slovak Research and Development Agency, APVV [SK-PL-18-0080, SK-PL-2015-0021, APVV-14-0267, SK-BY-RD-19-0019]
- Science Grant Agency [VEGA 1/0756/20]
- COST [CA17140]
- Polish National Agency for Academic Exchange, NAWA (Pl-SK 2019-2020) [PPN/BIL/2018/1/00150]
- Polish Ministry of Science and Higher Education
- NAWA International Academic Partnership Programme
- MINECO [CTQ2017-86224-P]
- Comunidad de Madrid (CAM) [IMMUNOTHERCAN-CM B2017/BMD-3733, NANO-DENDMED II-CM ref B2017/BMD-3703]
- Junta de Comunidades de Castilla-la Mancha (JCCM) [SBPLY/17/180501/000358]
- VI National RDi
- Plan 2008-2011
- Iniciativa Ingenio 2010
- Consolider Program
- CIBER Actions
- Instituto de Salud Carlos III
- European Regional Development Fund
This study investigated the complexation ability of dendronized gold nanoparticles with gp160 synthetic peptides derived from HIV envelope. Results showed that the nanoparticles interacted with HIV peptides, leading to structural changes, restricted mobility of attached fluorescence dye, and increased peptide helicity. Experimental results also indicated that dendronized gold nanoparticles could potentially serve as a platform for HIV peptide-based vaccination.
The unavailability of effective and safe human immunodeficiency virus (HIV) vaccines incites several approaches for development of the efficient antigen/adjuvant vaccination composite. In this study, three different dendronized gold nanoparticles (AuNPs 13-15) were investigated for a complexation ability with gp160 synthetic peptides derived from an HIV envelope. It has been shown that HIV peptides interacted with nanoparticles as evident from the changes in their secondary structures, restricted the mobility of the attached fluorescence dye, and enhanced peptide helicity confirmed by the fluorescence polarization and circular dichroism results. Transmission electron microscopy visualized complexes as cloud-like structures with attached nanoparticles. AuNP 13-15 nanoparticles bind negatively charged peptides depending on the number of functional groups; the fastest saturation and peptide retardation were observed for the most dendronized nanoparticle as indicated from dynamic light scattering, laser Doppler velocimetry, and agarose gel electrophoresis experiments. Dendronized gold nanoparticles can be considered one of the potential HIV peptide-based vaccination platforms.
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