期刊
JOURNAL OF PHYSICS D-APPLIED PHYSICS
卷 54, 期 12, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/1361-6463/abd0ae
关键词
atomic force microscopy; optical tweezers; Arp2/3; actin cortex; cell mechanics
资金
- Austrian Science Funds (FWF) [29562-N62]
- Slovenian Research Agency [P1-0055]
Cytoskeleton-disrupting drugs can have varied effects on cell mechanics depending on the deformation scales. The study found that actin-disrupting drugs CK-869 and jasplakinolide had different impacts on cellular stiffness, with CK-869 leading to decreased cell stiffness and jasplakinolide causing both stiffening and softening effects depending on the deformation scale. These results suggest that the effects of these drugs on cell mechanics are scale-dependent and vary based on the level of actin organization.
Cytoskeleton-disrupting drugs can have different effects on cell mechanics at different deformation scales. We therefore applied two complementary indentation techniques to study the effects of two actin-disrupting drugs on cellular stiffness of human umbilical vein endothelial cells. Optical tweezers were used to probe the cortical stiffness at small deformations, and atomic force microscopy was used to probe the bulk cell stiffness at larger deformations. The first drug studied was CK-869, which is an inhibitor of the actin branching complex Arp2/3, and has not been analysed yet in terms of mechanical effects. A significant decrease in cell stiffness upon treatment with CK-869 was measured with both techniques, which implies that actin branching is important for cell mechanics at small and large deformations. The second drug studied was jasplakinolide, for which ambiguous effects on cell mechanics have been reported. In line with previous studies, we found that jasplakinolide caused significant cell stiffening at large deformations but slight cell softening under small deformations. This result implies that jasplakinolide has different effects on different levels of actin organization.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据