4.5 Article

Discriminating between Concerted and Sequential Allosteric Mechanisms by Comparing Equilibrium and Kinetic Hill Coefficients

期刊

JOURNAL OF PHYSICAL CHEMISTRY B
卷 125, 期 1, 页码 70-73

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jpcb.0c09351

关键词

-

资金

  1. Minerva Foundation
  2. Federal German Ministry for Education and Research
  3. Kimmelman Center for Biomolecular Structure and Assembly

向作者/读者索取更多资源

Hill coefficients can be determined for equilibrium and transient kinetic data to provide insights into allosteric mechanisms. The ratio of equilibrium and kinetic Hill coefficients can indicate concerted or sequential transitions, with implications for symmetric dimers and homooligomers in ligand binding.
Hill coefficients, which provide a measure of cooperativity in ligand binding, can be determined for equilibrium (or steady-state) data by measuring fractional saturation (or initial reaction velocities) as a function of ligand concentration. Hill coefficients can also be determined for transient kinetic data from plots of the observed rate constant of the ligand-promoted conformational change as a function of ligand concentration. Here, it is shown that the ratio of the values of these two Hill coefficients can provide insight into the allosteric mechanism. Cases when the value of the kinetic Hill coefficient is equal to or greater than the value of the equilibrium coefficient indicate concerted transitions whereas ratios smaller than one indicate a sequential transition. The derivations in this work are for symmetric dimers but are expected to have general applicability for homooligomers.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据