期刊
JOURNAL OF PHYCOLOGY
卷 57, 期 3, 页码 742-753出版社
WILEY
DOI: 10.1111/jpy.13126
关键词
arabinogalactan protein; diffusible factor; Ectocarpus; gametophyte; life cycle; sporophyte
资金
- Centre National de la Recherche Scientifique
- European Research Council [638240]
- Sorbonne University
- French National Research Agency [ANR-10-BTBR-04-02]
- Chinese Scholarship Council [201608310119]
The life cycle of the filamentous brown alga Ectocarpus involves alternation between sporophyte and gametophyte generations, with deployment of the sporophyte developmental program requiring specific transcription factors. The sporophyte generation secretes a diffusible factor that may belong to the arabinogalactan proteins family.
The haploid-diploid life cycle of the filamentous brown alga Ectocarpus involves alternation between two independent and morphologically distinct multicellular generations, the sporophyte and the gametophyte. Deployment of the sporophyte developmental program requires two TALE homeodomain transcription factors OUROBOROS and SAMSARA. In addition, the sporophyte generation has been shown to secrete a diffusible factor that can induce uni-spores to switch from the gametophyte to the sporophyte developmental program. Here, we determine optimal conditions for production, storage, and detection of this diffusible factor and show that it is a heat-resistant, high molecular weight molecule. Based on a combined approach involving proteomic analysis of sporophyte-conditioned medium and the use of biochemical tools to characterize arabinogalactan proteins, we present evidence that sporophyte-conditioned medium contains AGP epitopes and suggest that the diffusible factor may belong to this family of glycoproteins.
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