期刊
JOURNAL OF ORTHOPAEDIC RESEARCH
卷 39, 期 10, 页码 2243-2251出版社
WILEY
DOI: 10.1002/jor.24964
关键词
fatty infiltration; gait analysis; muscle atrophy; shoulder impingement model; supraspinatus tendinopathy
类别
资金
- National Institutes of Health
- Orthopaedic Research and Education Foundation
- Virginia Toulmin Foundation
- Angiocrine Biosciences
This study induced shoulder tendinopathy in mice and found that muscle atrophy and fatty infiltration began at 6 weeks, with significant functional changes in gait identified as early as 4 weeks. Gait analysis provided key functional outcome measurements for evaluation of therapeutic strategies.
Muscle atrophy and fatty infiltration have been directly correlated with higher rates of incomplete or failed healing following surgical repair of the rotator cuff. The purpose of this study was to evaluate clinically relevant functional and morphological changes in the supraspinatus muscle at various time points in this model of rotator cuff tendinopathy. Subacromial impingement was induced in 47, male C57BL/6 mice (total 94 limbs) by implantation of a metal clip in the subacromial space. Specimens were evaluated at 4, 6, and 12 weeks postoperatively. Gait analysis was used to measure various kinematic parameters. Supraspinatus muscle wet weight, histology, and quantitative reverse-transcription polymerase chain reaction analysis of genes related to muscle atrophy and adipogenesis were performed to characterize the structural, cellular, and molecular changes. Muscle atrophy and fatty infiltration was evident beginning at 6 weeks, with progression out to 12 weeks. Gait analysis identified significant functional changes in many aspects of gait and abnormal stance tracing as early as 4 weeks, verifying alterations in upper extremity function. We have demonstrated that clinically relevant changes to the supraspinatus muscle are seen starting 6 weeks after induction of subacromial impingement. Furthermore, the gait analysis provides key functional outcome measurements that may be useful for future evaluation of new therapeutic strategies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据